Isolation, Purification and Immuno-Biochemical Characterization of Gir and Jersey Seminal Plasma Fertility Associated Proteins

Abstract

The objective of the present study was an attempt has been made to throw some light on the reproductive performance of Gir and Jersey bull in terms of evaluation of polypeptide characteristics of seminal plasma as well as mass activity, motility and morphology of spermatozoa. Seminal plasma from apparently normal semen containing viable sperm was collected for isolation; purification and immune-biochemical characterization of fertility associated proteins 26 kDa, 55 kDa, 14.3 kDa, 12 kDa and 16 kDa in bulls are a prominent immuno-dominant protein and have pivotal role in fertility. Research conducted in bovine seminal plasma showed that the abundance of specific protein in semen is related to fertility. Two seminal plasma protein 26 kDa and 55 kDa were more abundant in semen of high fertility bulls and two different proteins 16 kDa (pI 6.7) and 16 kDa (pI 4.1) were more abundant in lower fertility bulls. This paper reviews comparatively our current knowledge on the growing field of proteomics of the seminal plasma proteins and its relevance in relation to the in vivo situation, for the sake of reproductive biology, diagnostics and treatment. Proteins of the seminal plasma have an ample panorama of action and some appear responsible for establishing fertility.