Polymorphism of Exon 2-3 of Growth Hormone Gene in Surti and Mehsani Goats by PCR-RFLP

Jyotishree  Bayan; Vishnu Kharadi; Umed Ramani; Mamta Janmeda; Pawar Vallabh; Balkrishna Brahmkshtri

Authors

Jyotishree Bayan Department of Animal Genetics and Breeding , College of Veterinary Science, Navsari Agricultural University, Navsari, Gujarat, INDIA
Vishnu Kharadi Livestock Research Station, College of Veterinary Science, Navsari Agricultural University, Navsari, Gujarat, INDIA
Umed Ramani Department of Animal Biotechnology, College of Veterinary Science, Navsari Agricultural University, Navsari, Gujarat, INDIA
Mamta Janmeda Department of Animal Genetics and Breeding , College of Veterinary Science, Navsari Agricultural University, Navsari, Gujarat, INDIA
Pawar Vallabh Department of Animal Genetics and Breeding , College of Veterinary Science, Navsari Agricultural University, Navsari, Gujarat, INDIA
Balkrishna Brahmkshtri Department of Animal Genetics and Breeding , College of Veterinary Science, Navsari Agricultural University, Navsari, Gujarat, INDIA

Keywords:

Goat, Growth Hormone Gene, Polymorphism, PCR-RFLP

Abstract

The investigation was planned to study GH gene exon 2-3 polymorphism using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Surti and Mehsani goats. GH gene exon 2-3 region was found to be polymorphic on restriction digestion with Hae III which revealed two genotypes AA and AB with genotypic frequencies of 0.24 and 0.76 in Surti goats and 0.20 and 0.80 in Mehsani goats respectively. Both the population of Surti and Mehsani goats were not found to be in genetic equilibrium for GH locus exon 2-3 indicating selection pressure for growth. Polymorphism in exon 2-3 region of GH gene in both the goat breeds can be utilized in formulating breeding strategies based on marker assisted selection in both the goat breeds.