Investigation of ABCG2/PstI Polymorphism in Sahiwal Cattle by PCR-RFLP

Manvendra Singh; A. K. Gupta; I. D. Gupta; Arun Pratap Singh; Ashwani Arya; M. A. Mir

Investigation of ABCG2/PstI Polymorphism in Sahiwal Cattle by PCR-RFLP

Authors

Manvendra Singh Animal Science, Krishi Vigyan Kendra, Banda – 210001, Uttar Pradesh, INDIA https://orcid.org/0000-0002-1566-2264
A. K. Gupta DCB Division, ICAR-NDRI, Karnal - 132001 Haryana, INDIA
I. D. Gupta DCB Division, ICAR-NDRI, Karnal - 132001 Haryana, INDIA
Arun Pratap Singh Animal Science, Krishi Vigyan Kendra, Ajmer – 305206, Rajasthan, INDIA
Ashwani Arya Animal Science, Krishi Vigyan Kendra, Mahoba – 210423, Uttar Pradesh, INDIA
M. A. Mir DCB Division, ICAR-NDRI, Karnal - 132001 Haryana, INDIA

Keywords:

ABCG2 Gene, PstI, Polymorphism, PCR-RFLP, Sahiwal Cattle

Abstract

The present study was performed to investigate the polymorphism in the ATP-binding cassette superfamily G member 2 transporter (ABCG2) gene in Sahiwal cattle by PCR-RFLP assay. PCR-RFLP analysis of exon 14 was carried out using restriction enzyme PstI. PstI-RFLP for exon 14 revealed monomorphic pattern showing only one type of genotype, AA (292 bp) with genotypic frequencies of 1.0. AC and CC genotype were not observed in the studied Sahiwal population. The present study revealed that the selected Sahiwal population is monomorphic for ABCG2 gene as only single genotype (AA) was found in all the animals which were screened for studying polymorphism in ABCG2 gene.

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Published

31-08-2019

How to Cite

Singh, M., Gupta, A. K., Gupta, I. D., Singh, A. P., Arya, A., & Mir, M. A. (2019). Investigation of ABCG2/PstI Polymorphism in Sahiwal Cattle by PCR-RFLP. International Journal of Livestock Research, 9(8), 190–196. Retrieved from http://ijlr.org/ojs_journal/index.php/ijlr/article/view/1157