Pathology and Molecular Diagnosis of Chicken Infectious Anemia in Commercial Layers of Chhattisgarh

Prashant M. Sonkusale; Ratan Chnadra Ghosh; Devesh Kumar Giri; Vivek Kumar; Poornima Gumasta; Neha Shukla; Savita Bisen

Authors

Prashant M. Sonkusale Assistant professor, Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
Ratan Chnadra Ghosh Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
Devesh Kumar Giri Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
Vivek Kumar Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
Poornima Gumasta Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
Neha Shukla Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
Savita Bisen Department of Parasitology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA

Keywords:

CIA, Commercial Layers, Molecular Diagnosis, Histopathology

Abstract

Poultry is constantly exposed to various immunosuppressive agents such as viruses, mycotoxins, and environmental stress. Chicken Infectious Anemia (CIA) caused by a Circovirus is one of the very important viral diseases. During the present study, mortality of 15 to 25% due to Chicken infectious anemia in eleven, 6-11weeks old commercial layer flocks located at Durg, Rajnandgaon, and Raipur districts of Chhattisgarh was noticed which were not vaccinated against CIA. Characteristic clinical signs of anemia indicated by pale comb and wattle, yellowish changes in the bone marrow, and thymic atrophy were suggestive of CIA. Generalized lymphoid atrophy was the most constant and characteristic lesion found in CIA-affected birds. Clinical samples of thymus collected from eleven commercial layer flocks were confirmed as CIA by PCR amplification of 419bp of the VP2 gene of the virus. The clinical signs, gross and histopathological findings along with PCR amplification of the VP2 gene confirmed the outbreak of CIA in the commercial layer.

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