Metaphor Gel Electrophoresis: A Tool for High Resolution Genotyping with Microsatellite Marker in Pati; The Local Indigenous Duck of Assam

Purabi Kaushik; Kabita B. Kalita; R. J. Deka; Jnyanashree Saikia

doi:10.5455/ijlr.20200826061050

Authors

Purabi Kaushik College of Veterinary Science, Assam Agricultural University, Khanapara, Assam, INDIA
Kabita B. Kalita College of Veterinary Science, Assam Agricultural University, Khanapara, Assam, INDIA
R. J. Deka College of Veterinary Science, Assam Agricultural University, Khanapara, Assam, INDIA
Jnyanashree Saikia College of Veterinary Science, Assam Agricultural University, Khanapara, Assam, INDIA

DOI:

https://doi.org/10.5455/ijlr.20200826061050

Keywords:

MAGE, Microsatellite marker, Pati Duck, PCR

Abstract

Microsatellites are highly polymorphic, so they have been very useful in population genetics studies. High level of allelic variation, co-dominant mode of inheritance and potential for automated analysis make them an excellent tool for a number of approaches like genotyping, mapping, and genetic characterization. The resolution capability of Metaphor agarose gel is very fine. Study was conducted on 50 local pati ducks. Blood sample was collected from 5 different areas of Assam. Presently, a panel of 17 informative duck microsatellite markers were identified from database as reported by Alyethodi and Kumar (2010) and Huang et al. (2005). MAGE was successful in clearly separating the alleles with a size difference of very small difference. In microsatellite analysis for large number of samples MAGE can be considered very economical for analysis of polymorphisms.

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