Comparison of Three Tests for Diagnosis of Canine Parvovirus Infection in Himachal Pradesh

Abstract

The aim of present study is to evaluate the most sensitive and specific test for the diagnosis of canine parvovirus. For this three different tests like PCR, HA/HI and dot-ELISA were used to detect canine parvovirus infection. A total of 102 faecal samples were collected from the dogs having haemorrhagic gastroenteritis from different regions of Himachal Pradesh. The samples were tested by PCR, HA/HI and dot-ELISA diagnostic tests. Out of total 102 faecal samples collected from dogs having haemorrhagic gastroenteritis PCR detected 52 samples positive, dot-ELISA showed 49 samples positive s and HA/HI detected 41 samples positive. Therefore, percent positivity obtained by PCR was (50.98%), dot-ELISA was (48.03%) and HA/HI was (40.19%). Study concluded that PCR is the most important diagnostic test for the diagnosis of canine parvovirus and it is highly sensitive and specific test.