Morphometry of Feline Adipose Tissue Derived Mesenchymal Stem Cells in Culture

Shazia Nissar; Geetha Ramesh; T. A. Kannan; Sabiha Hayath Basha; Arunmozhi, N.; Rangasamy Seerangan; Shahid Hussian Dar

Authors

Shazia Nissar Department of Veterinary Anatomy, Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, INDIA
Geetha Ramesh Department of Veterinary Anatomy, Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, INDIA
T. A. Kannan Department Centre for Stem Cell Research and Regenerative Medicine, Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, INDIA
Sabiha Hayath Basha Department Centre for Stem Cell Research and Regenerative Medicine, Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, INDIA
Arunmozhi, N. Department of Gynaecology and Obstetrics, Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, INDIA
Rangasamy Seerangan Department of Gynaecology and Obstetrics, Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, INDIA
Shahid Hussian Dar Department of Veterinary Clinical Complex SKUAST- Kashmir, INDIA

Keywords:

Adipose Tissue and Culture, Feline, Mesenchymal Stem Cells, Morphometry

Abstract

Morphometric analysis of feline adipose derived mesenchymal stem cells (fAD-MSCs) was done at P1 and P2 (24 and 48 hours) of culture expanded using cover slip culture technique to record the morphometric details. In all the culture, spindle cells with long thin cytoplasmic processes were found to be more in number. The measure of length and width of cell and length and width of nucleus was done. There were no significant differences in both length and width of the cell and length and width of the nucleus between 24 and 48 hours of culture condition in P1 and P2.