Study of Virulence Genes, Antibiotic and β-Lactamase Profiles of Proteus mirabilis Isolated from Livestock and Livestock Products

Surya Teja Naidu; Bhavana Bodempudi; Bindu Kiranmayi Chinnam; Subhashini Nelapati; Srinivasa Rao Tumati; Nagaveni  Chekuri; Venkata Chaitanya Pedada

doi:10.5455/ijlr.20200714034333

Authors

Surya Teja Naidu Department of Veterinary Public Health and Epidemiology, N.T.R. College of Veterinary Science, Gannavaram, Vijayawada, Andhra Pradesh, INDIA
Bhavana Bodempudi Department of Veterinary Public Health and Epidemiology, N.T.R. College of Veterinary Science, Gannavaram, Vijayawada, Andhra Pradesh, INDIA
Bindu Kiranmayi Chinnam Department of Veterinary Public Health and Epidemiology, N.T.R. College of Veterinary Science, Gannavaram, Vijayawada, Andhra Pradesh, INDIA
Subhashini Nelapati Department of Veterinary Public Health and Epidemiology, N.T.R. College of Veterinary Science, Gannavaram, Vijayawada, Andhra Pradesh, INDIA
Srinivasa Rao Tumati Department of Veterinary Public Health and Epidemiology, N.T.R. College of Veterinary Science, Gannavaram, Vijayawada, Andhra Pradesh, INDIA
Nagaveni Chekuri Department of Veterinary Medicine, N.T.R College of Veterinary Science, Gannavaram, Andhra Pradesh, INDIA
Venkata Chaitanya Pedada Department of Veterinary Public Health and Epidemiology, N.T.R. College of Veterinary Science, Gannavaram, Vijayawada, Andhra Pradesh, INDIA

DOI:

https://doi.org/10.5455/ijlr.20200714034333

Keywords:

P. mirabilis, Virulotyping, Multiple antibiotic resistance index, β-lactamases, ESBLs, AmpC β- lactamases

Abstract

Proteus mirabilis is an emerging foodborne pathogen and has been associated with food poisoning outbreaks, urinary tract infections and causes serious diseases. The present study was undertaken to characterize P. mirabilis species from foods of animal origin based on cultural isolation, PCR confirmation, virulence profiles, antibiogram and production of β-lactamases. Among the 183 samples (135 foods of animal origin and 48 chicken cloacal swabs), 61 (33.33%) isolates were found to be P. mirabilis by species-specific PCR. All the P. mirabilis isolates carried different combinations of putative virulence genes. β-lactamase genes were detected in a total of 37 isolates out of 61(60.65%) with blaTEM being the predominant gene detected (89.18%) followed by blaOXA (10.81%), blaSHV (5.40%) and DHA (2.70%). The findings of this study revealed that there may be a danger of transmission of drug resistant P. mirabilis to consumers through consumption of improperly cooked meat which can lead to treatment failure.