Isolation, Characterization of Lachnospira pectinoschiza from Goat Rumen and Effect of Addition on in vitro Fermentation Parameters

Abstract

Isolation of pectinophile bacteria Lachnospira pectinoschiza from goat rumen was carried out. Their molecular characterization was done after amplification of 16s rDNA on the basis of gene sequence using Ez Bio Cloud database. Isolated culture was evaluated in In vitro gas production test for their effect on gas production, methane production, in vitro true digestibility and volatile acid fraction on high (100% gram straw) and medium (50% Gram straw and 50% concentrate mixture) roughage ration. There was no significant effect of Lachnospira pectinoschiza culture on the in vitro gas production, methane production as well as in vitro true digestibility. Total volatile fatty acids and their fractions were also similar among all the groups. The effect was similar on both type of ration. However lower methane production and higher digestibility was reported with medium roughage ration. The present study concluded that Lachnospira pectinoschiza isolated from rumen of goat has no significant effect of on in vitro rumen fermentation metabolites on high and medium roughage ration.