The aim of present study is to estimate the normal values of blood parameters and to study the role of sex on serum biochemical values and haematological profile in adult emus. Investigations were carried out on 8 male and 8 female adult emu birds at private emu farm, Tirupati, Chittoor District, Andhra Pradesh, India. The experimental birds are kept under scientific management, housing and feeding practices. The serum biochemical values like total protein, cholesterol, glucose, creatinine and urea were estimated using commercial kits. There is a significant increase (P<0.05) in Total protein and Creatinine in females than males, while the haematological parameters viz., total RBC count, total WBC count, Haemoglobin, PCV, ESR were estimated using standard protocol, erythrocyte indices - Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH) and Mean Corpuscular Haemoglobin Concentration (MCHC) were measured. The males had significantly (p<0.05) higher value of total RBC count and ESR than females. Statistical analysis of the data was analyzed by one way ANOVA.