A study was conducted to evaluate the effect of dietary supplementation of organic chromium on carcass characteristics and haemato-biochemical profile in broiler chickens. Day old, 200 broiler chicks were divided into four treatment groups with replicates of 10 chicks in each and were assigned into four iso-nutritive diets, viz., T1: Control diet (feed without organic chromium (CrPro) supplementation), T2: Control diet supplemented with CrPro @ 400 µg/kg feed, T3: Control diet supplemented with CrPro @ 800 µg/kg feed and T4: Control diet supplemented with CrPro @ 1200 µg/kg feed. The feeding experiment was carried out for 42 days. Dietary supplementation of organic chromium in broiler chicken diet had non-significant (P>0.05) effect on carcass traits, dressing percentage and weights of liver, heart, gizzard, kidney and spleen in broiler chickens. No significant (P>0.05) effect was found in levels of haemoglobin, packed cell volume, total leukocyte count and total erythrocyte count by the organic chromium supplementation. Significant (P<0.05) reduction in concentrations of serum glucose, triglycerides and total cholesterol were observed in organic chromium supplemented groups. Based on results, it was concluded that organic chromium supplementation in broiler chickens reduced serum levels of glucose, triglycerides and total cholesterol, but did not affect carcass traits and haemato-biochemical parameters.