Indigenous Corrosion Cast Preparation of Biological Organs using Denture Material

Abstract

Cost effective indigenous ‘Corrosion cast’ method was devised to prepare 3D details of biological organs. Thirty paired fresh kidneys of 10 each from three species i.e., cattle, goat and pig were utilized to study the details of renal collecting system (RCS) and blood vessels. Organs were flushed with warm normal saline solution through their ureters after which a cold cure self-polymerizing acrylic powder and solution mixed in a 1:2 ratio along with few drops of desired colored dye was injected. Specimens were effectively polymerized at room temperature, followed by their immersion in 75% HCl till corrosion process was completed. Later the specimens were dissected to study the branching pattern of ureter in cattle and renal pelvis in pig and goat. Specimens were kept in museum without any preservatives for long time and used for teaching the anatomy of vessels, ducts and cavities of organs. This technique reduces the biological risk present in formalin fixed organs.