Phylogenetic Analysis of Non Structural Protein in Peste des petits ruminants (PPR) Virus

P. Kirthika; Mihir Sarkar; Deepak Kumar; Ajay Kumar; Kaushal Kishor Rajak; Praveen Singh

Authors

P. Kirthika Division of Animal Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, INDIA
Mihir Sarkar Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, INDIA
Deepak Kumar Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, INDIA
Ajay Kumar Division of Animal Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, INDIA
Kaushal Kishor Rajak Division of Biological Products, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, INDIA
Praveen Singh CIF Bioengineering and Biophysics Section, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, INDIA

Keywords:

PPRV, Non-Structural Protein, V Protein, Phylogeny

Abstract

Peste des petits ruminants (PPR) is an acute, highly contagious, and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality. The PPR virus virion exhibits 6 major proteins, on surface, nucleocapsid. The proteins play an important role in viral pathogenesis and its survivability in host. The protein inherits structure to function relationship and needs to be studied closely. The genetic diversity of non-structural V protein encoding region of P gene (894bp) of PPR virus was accomplished by using phylogenetic analysis of different strains of PPRV. Analysis of the sequence revealed that it could demonstrate the genetic diversity among various strains of PPRV. Based on the V protein tree, the PPRV was categorized into three different clades each representing different lineages. The data could be validated for the survey of viral dynamics, epidemiological studies as well as prevention and control of the disease.