Pathology and molecular detection of Salmonellosis in commercial chickens of Chhattisgarh

Prashant Sonkusale; Ratan Chandra Ghosh; Devesh Kumar Giri; Vivek Kumar; Poornima Gumasta; Neha Shukla; Savita Bisen

Authors

Prashant Sonkusale Assistant professor, Department of Pathology, College of Veterinary Science & A. H. Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh INDIA
Ratan Chandra Ghosh Department of Pathology, College of Veterinary Science & A. H. Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh INDIA
Devesh Kumar Giri Department of Pathology, College of Veterinary Science & A. H. Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh INDIA
Vivek Kumar Department of Pathology, College of Veterinary Science & A. H. Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh INDIA
Poornima Gumasta Department of Pathology, College of Veterinary Science & A. H. Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh INDIA
Neha Shukla Department of Pathology, College of Veterinary Science & A. H. Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh INDIA
Savita Bisen Department of Parasitology, College of Veterinary Science & A. H. Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh INDIA

Keywords:

Commercial Chickens, Pathology, Molecular Diagnosis, Salmonellosis

Abstract

Avian salmonellosis is an important disease-causing serious impediment to the development of the poultry industry especially in developing countries. During the present study, mortality of 16 to 22% due to Salmonellosis in thirteen, 1-2 weeks old commercial chicken flocks located at Durg, Rajnandgaon, and Raipur district of Chhattisgarh was noticed. Characteristic clinical signs of depression, anorexia, huddling, droopy wings, dehydration, laboured breathing, diarrhoea, and adherence of faeces to the vent were suggestive of Salmonellosis. Necrotic foci on the liver, omphalitis, and catarrhal enteritis were the characteristic lesions found in Salmonella-affected birds. Colorless colonies on MLA, light pink colonies on BGA, and jet-black colonies on XLD agar confirmed the Salmonella infection. Clinical samples of liver collected from thirteen commercial chicken flocks were further confirmed as Salmonella spp. by PCR amplification of 423bp of InvA gene. The clinical signs, lesions, and cultural characteristics along with PCR amplification of InvA gene confirmed Salmonella infection in commercial chickens of Chhattisgarh.

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