In Vitro Capacitation of Boar Spermatozoa: Role of Heparin

Ainul Haque; Kutubuddin Ahmed; Dhireswar  Kalita; Shantanu Tamuly; Dibyajyoti Talukdar

doi:10.5455/ijlr.20180720060946

Authors

Ainul Haque Department of A.R.G.O., College of Veterinary Science, Khanapara, Assam, INDIA
Kutubuddin Ahmed Department of A.R.G.O., College of Veterinary Science, Khanapara, Assam, INDIA
Dhireswar Kalita ICAR- AICRP on Pig, Assam Agricultural University, Khanapara, Assam, INDIA
Shantanu Tamuly Department of Veterinary Biochemistry, College of Veterinary Science, Khanapara, Assam, INDIA
Dibyajyoti Talukdar Department of A.R.G.O., College of Veterinary Sciences & Animal Husbandry, Aizawl, Mizoram, INDIA

DOI:

https://doi.org/10.5455/ijlr.20180720060946

Keywords:

Functional Integrity, Hampshire Boar Spermatozoa, Heparin, In-Vitro Capacitation

Abstract

The aim of this study was to evaluate heparin induced in vitro capacitation associated changes in spermatozoa of crossbred Hampshire boar. Therefore, freshly ejaculated and washed spermatozoa of 4 Hampshire boar were in vitro capacitated in TALP medium supplemented with BSA, heparin and hepes buffer at a concentration of 6 × 10⁹ spermatozoa/ml at 37 ºC for 5 hours. Capacitation status of spermatozoa in terms of the hyperactivated motility, acrosome membrane integrity, total hypomostic swelling test (HOST) reacted spermatozoa, sperm membrane protein (SMP) and cholesterol content were estimated for each ejaculate at 1 hour interval starting from 0 to 5 hours of incubation. The results revealed that the highest hyperactivation of spermatozoa (71.86 ± 1.55%) was recorded at 4 hours of incubation while the percentage of detached acrosome, HOST reacted spermatozoa, SMP, cholesterol levels decreased significantly (P < 0.01) with increasing period of incubation. In conclusion, heparin induces in vitro capacitation changes in the boar spermatozoa as evident by highest hyperactivation of spermatozoa at 4 hours of incubation.