Study has been carried out on the animals suffering from clinical bovine babesiosis in the Patna districts of Bihar, India. A total number of 36 lactating cows of different breed and ages were included in the study for the effect of babesia infection on oxidant marker, antioxidant status, trace mineral status and mean corpuscular fragility of RBC. Erythrocytic LPO in clinically affected cattle were significantly (P<0.05) higher than healthy animals. GSH, SOD and catalase values in ailing animals were significantly (P<0.05) lower than healthy animals. Hemoglobin concentration was significantly (P<0.05) lower in ailing animals. Trace elements Zn, Cu & Se were also significantly reduced whereas serum iron found to be significantly (P<0.05) increased due to hemolysis in infected cows. Erythrocyte fragility at 0.9%, 0.8%,0.4% and 0.2% NaCl concentration was significantly (P<0.05) higher than the control group. However, there was lower erythrocytic fragility in 0.6% concentration of NaCl in infected animals. In conclusion, anemia occurs in Babesia bigemina infection is due to corpuscular oxidative damage as revealed from lipid peroxidation, antioxidant status and associated trace minerals deficiency contributing to RBC fragility and intravascular hemolysis.