High blood pressure is a chronic disease known as “silent killer”. BAY 41-272 is showing antihypertensive and cardio-protective activity. Present study was planned to analyse the effect of BAY 41-2272 on Mean Arterial Pressure, biochemical parameters and histomorphology of hypertensive rats. Male wistar rats were divided in three groups namely control (group-A), L-NAME induced hypertensive rats (group-B) and BAY 41-2272 treated L-NAME hypertensive rats (group-C). Group-A was treated with normal saline solution intramuscularly for last three days. However, group-B was treated with both last three days of saline solution and 42 days L-NAME (50mg/kg) administration from day one orally dissolving in drinking water. Whereas, group-C followed same treatment protocol of group-B and in addition BAY 41-2272 was administered daily for last 3 days (day 40-42) at two different dosages (1 & 3mg/kg) intramuscularly dissolving in saline solution. Three days BAY treatment significantly ameliorate biochemical parameters and improves histomorphology of BAY treated rats compared to untreated hypertensive rats.