Kidneys: Histopathological Changes in Diclofenac Induced Visceral Gout in Broilers and its Amelioration with Natural Herbals

Abstract

A study was conducted to examine the gross and histopathological changes in the kidneys of diclofenac induced visceral gout in day old broiler chicks and its amelioration with an ayurvet product. A total of 125 healthy day-old male broiler chicks (Vencobb strain) were divided into 5 groups consisting of 25 birds in each. The group 1 birds served as control and group 2 served as diclofenac toxic control (@ 30 ppm in feed) for 14 days. Group 3 birds were treated with ayurvet product (AV/AUP/16 @ 5 mL/day/100 birds for 0-2 weeks, 10 mL/day/100 birds for 2-4 weeks, 20 mL /day/100 birds for 4-6 weeks) upto 42 days. Group 4 were treated with diclofenac for 14 days along with ayurvet product from 1^st to 42^nd day. Group 5 birds were treated with diclofenac for 14 days followed by ayurvet product from 15^th day to 42^nd day. These birds were sacrificed on 14^th, 28^th and 42^nd day and observed pale colored, enlarged kidneys in diclofenac treated group birds and presence of light pink coloured urate crystals as radiating pattern in kidney parenchyma (H&E stain). These urate crystals were black in color with De Galantha’s stain. The histopathological changes were reduced in ayurvet product treated groups.