Pathological Changes and Molecular Detection of Avian Nephritis Virus from Commercial Broiler Chicken

M. Sathiyaseelan; R. Kumar; A. W.  Lakkawar; M. G. Nair; J. Thanislass; K. C. Varshney

Authors

M. Sathiyaseelan Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, INDIA
R. Kumar Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, INDIA
A. W. Lakkawar Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, INDIA
M. G. Nair Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, INDIA
J. Thanislass Department of Biochemistry , Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, INDIA
K. C. Varshney Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, INDIA

Keywords:

Avian Nephritis Virus, Broiler Chicken, Gout, Pathology, RT-PCR

Abstract

Gout is a metabolic disorder that occurs due to multiple causes including management, nutrition and infection. Avian nephritis virus (ANV) has been associated with nephropathy in commercial broilers. Hence the present study reports detection of ANV by reverse transcriptase polymerase chain reaction (RT-PCR) and its associated pathomorphology in gout affected commercial broiler from Puducherry and Tamilnadu during March 2015 and October 2015. Gout was recorded grossly in 24/120 (20%) and microscopically in 28/120 (23.33%) cases. Out of the 18 samples screened for ANV, 2 (11.11%) samples were found to be positive by RT-PCR. On microscopical examination of kidney moderate to severe dilatation of tubules and focal to multifocal interstitial mononuclear cells infiltration were observed. Isolation and identification of ANV is complicated and time consuming, hence the RT-PCR used in the present study can be a potential diagnostic tool for screening of ANV in the cases of gout and nephropathy in chickens.