Detection of Polymorphism in Coding Region of SLC11A1 Gene in Jersey Crossbred Cattle

M. Malarmathi; N. Murali; R. Saravanan; P. Gopu; L. Gunaseelan

Authors

M. Malarmathi Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal, Tamil Nadu, INDIA https://orcid.org/0000-0003-3279-3810
N. Murali Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal, Tamil Nadu, INDIA
R. Saravanan Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal, Tamil Nadu, INDIA
P. Gopu Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal, Tamil Nadu, INDIA
L. Gunaseelan Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal, Tamil Nadu, INDIA

Keywords:

Jersey Crossbred Cattle, NRAMP1, SNP, SLC11A1, Transversion

Abstract

SLC11A1 gene (Solute Carrier 11 A1 gene), formerly called as Natural Resistance Associated Macrophage Protein 1 (NRAMP1) plays a critical role in innate immunity and mutation of this gene is responsible for susceptibility to a number of intracellular pathogens. The polymerase chain reaction - restricted fragment length polymorphism (PCR-RFLP) analysis (Pst I) of exon 11 in 52 Jersey crossbred cattle revealed presence of three genotypes CC (231bp), CG (231 bp, 200 bp, 31 bp) and GG (200 bp, 31 bp) confirming C > G transversion in coding region. The genotypic frequencies in pooled samples were estimated as 0.24 (CC), 0.48 (CG) and 0.28 (GG). This polymorphism observed in exon 11 is due to single nucleotide polymorphism (C > G). However, no polymorphic pattern was observed in exonic regions 4-5 and 5-6.