Effect of Iron Restriction on the Whole Cell and Outer Membrane Proteins of Riemerella anatipestifer Isolates of Kerala

Abstract

Globally, riemerellosis is caused by Riemerella anatipestifer, has a major concern, especially around the duck producing countries in variable degrees. A contemplate was led to access the homogeneity/heterogeneity between the Riemerella isolates (RA1 and RA2) by comparing the whole cell and outer membrane protein profiles in iron restricted and repleted condition. On Sodium dodecyl sulphate polyacrylamide gel electrophoretic analysis, both the isolates under iron deprived condition, commonly increased the production of outer membrane proteins, 51 and 75 kDa, among the five increased proteins in each. Also, among the increasingly produced eight and six whole cell proteins of RA1 and RA2, respectively, 38 and 51 kDa were present in both under iron depleted condition. The present study showed heterogeneity in the protein profile of Riemerella isolates, RA1 and RA2. Both the isolates, under iron restricted conditions, not only up and down regulate various whole cell and Omp proteins, but also few novel proteins had been produced in iron depleted environment.