Effect of Intron 3 and Exon 5 Polymorphism in GH1 Gene on Milk Production and Milk Composition Traits in Karan Fries (Holstein Friesian Crossbred) Cattle

Abstract

The present study was undertaken to study the effect of SNPs in intron 3 and exon 5 region of GH1 gene on milk production and milk composition traits in Karan Fries (HF crossbred) cattle. DNA was isolated from the blood samples of 78 pedigreed animals and PCR amplification was done, followed by PCR-RFLP. The phenotypic data was adjusted for non- genetic factors and regression analysis was done. Multiple regression of SNPs in intron 3 and exon 5 region of GH1 gene revealed that on an average, test day milk yield increased by 1.199 kg, test day fat yield by 51 gms and test day SNF yield increased by 113 gms. Both of these SNPs could be considered as potential genetic markers for early selection of animals based on test day milk production and milk composition traits and could be incorporated in the Marker Assisted Selection strategy in future.