Variation in Serum Immunoglobulin, Histamine, and Cytokines Response between Resistant Garole and Susceptible Sahabadi Sheep Infected with Haemonchus contortus

Gautam Bordoloi; Anupam Brahma; Supradip Das; Ananta Hembram; Saroj Rai; Ruma Jas

Authors

Gautam Bordoloi Lakhimpur College of Veterinary Science, Saboti Road, 9/6 Koilamari, Assam, 787 051 INDIA
Anupam Brahma Faculty of Veterinary and Animal Science, Institute of Agricultural Science, RGSC, BHU, Mirzapur 231307, Uttar Pradesh, INDIA
Supradip Das Department of Veterinary Parasitology, West Bengal University of Animal and Fishery Sciences, 37 K. B. Sarani, Kolkata 700 037, West Bengal, INDIA https://orcid.org/0009-0009-5520-0847
Ananta Hembram College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar - 751003, INDIA
Saroj Rai National Dairy Research Institute-Eastern Regional Station, Kalyani, Nadia 741 235 West Bengal, INDIA https://orcid.org/0000-0002-1250-1342
Ruma Jas Department of Veterinary Parasitology, West Bengal University of Animal and Fishery Sciences, 37 K. B. Sarani, Kolkata 700 037, West Bengal, INDIA https://orcid.org/0000-0003-3502-3993

Keywords:

Garole Sheep, Host Resistance, Haemonchus contortus, Immune Response

Abstract

The immune response of resistant Garole sheep to Haemonchus contortus infection was compared with susceptible Sahabadi sheep. Each breed of sheep was divided into infected (n = 6) and control (n = 6) groups, and the infected groups were orally infected with H. contortus larvae. Faecal egg counts (FEC), and concentrations of immunoglobulin (IgA and IgE), histamine and cytokines (IFN-γ and IL-4) in serum were measured in all the experimental sheep. Faecal egg count was significantly (P < 0.05) lower in Garole sheep compared to Sahabadi sheep. Serum IgA and histamine concentration increased (P < 0.05) in resistant Garole sheep compared to Sahabadi sheep, with a non-significant difference in IgE concentration. Significantly (P<0.05) greater levels of serum IFN-γ and IL-4 were observed in resistant Garole sheep than in the susceptible Sahabadi sheep. Therefore, it can be concluded that these immunological parameters played an important role in the comparative resistance of Garole sheep.