Microscopic and Electron Microscopic Studies of Malassezia pachydermatis

Abstract

The Malassezia pachydermatis was isolated from clinical cases presented with signs of recurrent, moist, seborrhoeic dermatitis and transferred to sabourads dextrose agar. After several subcultures, pure colonies of the yeast were acquired which was confirmed under under high power field. The colonies were soft cream coloured and where as yeast appeared as dark blue coloured, foot print shaped when stained by diff quick method. Further, under electron microscope, the malassezia organism appeared as unique peanut or globose to ellipsoid in shape, round at one end and blunt at the other on SEM (Scanning electron microscope) and multilayered, thick cell wall with nucleus and vacuoles of various sizes and mitochondria of various shape and size and with bulgings between mother and daughter cell which was measuring about 3.19 to 4.56 µ in size, were the significant findings noticed on transmission electron microscope.