Phytochemical Analysis and Antibacterial Activities of Azadirachta indica against Pathogens Isolated from Bovine Subclinical Mastitis

Abstract

The phytochemical components of leaves of Azadirachta indica showed the presence of saponin, tannin and terpenoids and the absence of alkaloids, reducing sugars, glycosides and flavanoids in the methanolic extract and the presence of alkaloids, reducing sugars and terpenoids and the absence of saponin, tannins, glycosides and flavanoids in hydromethanolic extract. The test organisms were susceptible to concentrations higher than 50 mg / ml of the plant extracts. The MIC of methanolic extract of Azadirachta indica leaves was recorded to be 15.62 mg / ml for S. aureus and 31.25 mg / ml for both S. agalactiae and E. coli. The hydromethanolic extract of A. indica leaves showed MIC of 31.25 mg / ml for S. aureus, S. agalactiae and E. coli.  The highest recovery rate was seen in a group, treated with intramammary infusion of enrofloxacin BID for a period of 3 days as compared to other treated group.