Evaluation of Anti-Inflammatory and Antihypertensive Activities of Bioactive Peptides Derived from Trypsin Treated Cow Milk Casein

Santosh  Kumar; Bilkess Nabi; Ashwani Kumar  Sanghi; Jafar Khaliq  Lone

doi:10.5455/ijlr.20200606043113

Authors

Santosh Kumar MDETT Research Center, BAIF Development Research Foundation, Dharoli, Jind, Haryana, INDIA
Bilkess Nabi Dolphin PG Institute of Biomedical & Natural Sciences, Dehradun, Uttarakhand, INDIA
Ashwani Kumar Sanghi Dolphin PG Institute of Biomedical & Natural Sciences, Dehradun, Uttarakhand, INDIA
Jafar Khaliq Lone Dolphin PG Institute of Biomedical & Natural Sciences, Dehradun, Uttarakhand, INDIA

DOI:

https://doi.org/10.5455/ijlr.20200606043113%20%20%20%20

Keywords:

Angiotensin Converting Enzyme (ACE), Antihypertensive, Anti-Inflammatory, Bioactive Peptides, Erythrocyte Membrane Integrity, Trypsin

Abstract

Cow milk proteins are considered as the rich source of bioactive peptides which has a great influence in the various biological processes. The present study was aimed to evaluate anti-inflammatory (stabilizing of Erythrocyte Membrane Integrity) and antihypertensive (Angiotensin Converting Enzyme inhibition) bioactive peptides derived from cow milk protein casein after treatment with trypsin. Casein was prepared and treated with trypsin & extent of hydrolysis was analyzed. Bioactive peptides thus generated were evaluated for their said activities. Maximum anti-inflammatory effect was shown after 4 hours of enzymatic treatment; while maximum antihypertensive effect was shown after 3 hours of treatment. The results obtained hence proved that bioactive peptides released during tryptic digestion at different incubation times show significant effect on metabolic activities providing the need for further investigation for their targeted use as human nutritional supplements for specific medical conditions.