A study was carried out to find out the chemical composition, mineral profile, Cornell net carbohydrate and protein system (CNCPS) fractionations and in vitro digestibility of nutrients among different samples of corn dried distiller grain solubles (DDGS), collected from five ethanol processing industries of Andhra Pradesh. The mean CP, CF, EE, and TA of various DDGs samples were, 28.92, 7.68, 11.86, and 5.75, respectively. Similarly the calcium, phosphorous, sodium, potassium, magnesium and sulfur content of DDGs were 0.3, 0.61, 0.32, 0.82, 0.27, and 0.78, respectively. CNCPS fractionations revealed that DDGS can be effectively used as superior quality carbohydrate and protein source. The average in vitro digestibility coefficients of DM, CP, NDF and ADF were, 71.45, 78.96, 70.42, and 63.36%, respectively. Significant difference among the nutrient digestibility of samples studied by in-vitro digestibility and CNPS fractionation may be attributed to difference in processing procedures followed by the industrial plants.