An investigation was done to study the diagnostic parameters in dogs affected with Exocrine Pancreatic Insufficiency (EPI). 76 dogs presented with the history of passing loose, voluminous, grey coloured greasy faeces were selected for the investigation. Faecal samples of all 76 dogs were screened for parasitic ova and all were found negative and further screened for the presence of trypsin, fat and protein by standard procedures. Among these, 18 dogs (23.68%) were negative for faecal trypsin and presence of fat, starch and protein were noticed in 34 (44.74%), 31 (40.79%) and 25 (32.89%) respectively. Further haemato biochemical estimations were done and 23.68 percent (18) tested were found positive for EPI and are divided in to 3 groups of 6 animals in each and given different therapeutic regimens. All the affected dogs showed no significant change in haematological parameters in all three groups and among biochemical parameters, serum glucose and serum GGT were found to be normal but serum Amylase and serum Lipase levels were found to be decreased. After therapy a significant (p<0.05) improvement was noticed. According to the findings, x-ray film digestion test, microscopic examination of faeces for the presence of fat, starch and protein; decreased serum amylase and lipase were shown to be effective for the diagnosis of EPI in canines.