The study was conducted on ten dogs presented with diaphyseal fracture of long bones. All the fractures were repaired by titanium elastic stable intramedullary nailing. To study the haemato-biochemical profile of the dogs blood samples were collected in EDTA and serum collecting vials at different time intervals i.e., pre-operative, immediate post-operative, on 15th, 30th and 60th post-operative days. There were statistically significant variations in the mean values of total leucocyte count, neutrophils (%), lymphocytes (%), platelet count, serum calcium and sodium ion levels during the different time intervals of observation. But clinically, all the haematological and serum biochemical parameters were within the normal range for dogs. So, it was inferred that application of titanium elastic stable intramedullary nailing does not cause any systemic adverse effects in dogs.