The A feeding trial was conducted for a period of 12 weeks duration to evaluate the effect of graded levels of pudina leaf powder supplementation on serum biochemical parameters in laying hens. A total number of 120 White leghorn laying hens of 36 weeks old were randomly divided in a completely randomized design into 4 treatment groups with 6 replicates of 5 birds each. The laying hens of treatment groups T1, T2, T3 and T4 were supplemented with feed containing 0, 5.0, 7.5 and 10.0 g/kg of pudina leaf powder respectively. The trial was conducted for a duration of 12 weeks (36 -47 weeks age group). At the end of trial, blood samples were collected to study serum-biochemical parameters. The results of the experiment revealed that serum total protein, albumin, globulin and HDL-cholesterol were significantly (P<0.05) increased and the concentration of serum glucose, total cholesterol, LDL- cholesterol, VLDL-cholesterol, triglycerides, uric acid, AST and ALT were significantly (P<0.05) decreased in groups supplemented with pudina leaf powder. The overall best performance was recorded in treatment group supplemented pudina leaf powder at 10.0 g/kg. It can be concluded that pudina leaf powder supplementation improves serum biochemical levels with no adverse effect on liver and kidney functions.