The present study was aimed to assess the current epidemiological status of bovine tick-borne diseases (TBDs) in and around Chennai by conventional staining method and polymerase chain reaction (PCR). A total of 154 blood smear and whole blood samples were screened by Leishman staining and PCR respectively. PCR assay (50.65%) revealed significantly higher sensitivity in detection of TBDs in clinically suspected cattle than microscopic examination (35.06%). Results showed that Anaplasma spp. (20.78%) was the most prevalent parasite of cattle followed by Theileria spp. (11.69%) and Babesia spp. (2.6%) in Chennai. The infection of Anaplasma spp. (27.77 %), Babesia spp. (5.55 %) and Theileria spp. (11.11 %) were higher in less than 2 year of age group. The prevalence of anaplasmosis was relatively high (21.32%) in cross-bred cattle. However, higher prevalence of babesiosis (5.55%) and theileriosis (22.22%) was noticed in non-descript animals. Statistical analysis using chi-square test indicated a highly significant (P < 0.01) difference between these tests.