A study was conducted to evaluate the seasonal influence (peak winter and summer) and the efficacy of three extenders (egg yolk based TFYG extender and egg yolk free soya bean based commercial extenders Optixcell and Andromed) on quality and freezability of Surti buffalo bull semen in middle Gujarat. Nutritionally the bulls were managed identically throughout the year. Semen ejaculates (6/bull/season, total 36) revealed mean ejaculate volume 3.23±0.18 ml, sperm concentration 963.06±64.74 million/ml, progressive motility 79.94±0.83 %, live sperm 83.67±0.62 %, abnormal sperm 7.69±0.41 %, and sperm with intact plasma membrane 83.19±0.75 % and intact acrosome 94.92±0.24 %. Only the progressive sperm motility (80.56±0.98 vs. 73.33±0.57 %) and HOST (85.33±0.81 vs 81.06±1.07 %) were significantly (p<0.05) higher with lesser sperm abnormality (6.17±0.53 vs. 9.22±0.37 %) during winter than in summer. Semen samples split diluted with TFYG, Optixcell and Andromed extenders revealed the overall mean values (%) of progressive sperm motility, livability, abnormality, plasma membrane integrity and acrosomal integrity during winter season as 75.09±0.49, 76.41±0.51, 5.52±0.21, 77.22±0.38 and 92.61±0.28 at pre-freeze stage and 42.13±1.25, 54.81±0.88, 8.46±0.29, 33.26±0.96 and 90.78±0.28 at post-thaw stage, respectively. The respective values (%) in summer season were 65.37±0.53, 71.56±0.50, 9.81±0.28, 68.17±0.96 and 92.81±0.28 at pre-freeze stage, and 33.43±0.80, 50.78±0.76, 14.46±0.29, 28.04±0.78 and 90.43±0.29 at post-thaw stage. The overall mean sperm post-thaw motility/longevity at 0, 30, 60 and 120 min of incubation at 37°C was 43.13±1.34, 36.72±1.41, 29.47±1.42 and 19.83±1.34 % during winter season and 34.73±0.81, 29.31±0.83, 21.86±0.69 and 14.34±0.82 % in summer season, respectively. The initial quality as well as freezability of semen in terms of motile, live, normal and HOS reactive sperm including post thaw longevity were significantly (p<0.01) better in winter season than in summer season. Further, the values of all the five semen quality parameters studied were comparatively better in Optixcell than TFYG and Andromed extenders with significant differences mainly in sperm progressive motility, viability and post-thaw longevity in both the seasons. However, the season x extender interaction was not significant for any of the sperm quality parameters studied. Winter was the best season for harvesting optimum quality and freezability of buffalo semen in Optioxcell extender.