Serological Detection of Mycobacterium bovis from Suspected Cattle by Fluorescence Polarization Assay

Rabyia Javed; Deepti Narang; Mudit Chandra; S. T. Singh; G. Filia

Authors

Rabyia Javed Department of Veterinary Microbiology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences, Ludhiana, Punjab, INDIA
Deepti Narang Department of Veterinary Microbiology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences, Ludhiana, Punjab, INDIA
Mudit Chandra Department of Veterinary Microbiology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences, Ludhiana, Punjab, INDIA
S. T. Singh Directorate of Livestock Farms, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences, Ludhiana, Punjab, INDIA
G. Filia ADRC, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences, Ludhiana, Punjab, INDIA

Keywords:

Antibody, CID, FPA

Abstract

A Fluorescence polarization assay (FPA) was optimized and validated to detect antibodies to Mycobacterium bovis in cattle sera. A total 192 (cattle and buffalo) blood samples were screened for the CMI response by Comparative Intradermal (CID) tests and Gamma interferon (IFN-γ) tests as per the standard protocol of OIE, World Organization of Animal Health 2009. The presence of antibodies is indicative of prior infection with Mycobacterium bovis. Out of 192 animals screened, 14 and 23 animals were positive by CID and IFN-γ respectively. The diagnostic test revealed the results as out of 33 samples ( positive samples by either of the two tests) and subjected to FPA, 7 samples were positive by confirmatory protocol given by the kit (milli-polarisation >50). A total of 53 animals (33 of which were positive by either of the CID and IFN tests, while 20 were negative by either of the CID and IFN-γ tests) were subsequently selected for the FPA. The FPA detected 12/53 (22.64%).