Isolation and Identification of Bacterial Organisms from Cattle with Acute Respiratory Infections in Punjab, India

Abstract

A study was undertaken on bovines affected with acute respiratory infections following lumpy skin disease (LSD) epidemics in 2022 to identify the causative organisms. Nasal swabs and blood samples (n=40) of cattle with acute respiratory disease from veterinary hospitals and dairy farms in and around the Ludhiana region were collected. The clinical samples were initially processed for bacterial isolation using blood agar and brain heart infusion agar. For confirmation, the organisms were subjected to Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis. The study revealed the presence of several bacterial organisms viz., Mannheimia varigena, Escherichia coli, Corynebacterium (C. xerosis, C. stationis and C. camporealensis), Acinetobacter (A. towneri, A. boumanii, A. indicus), Pseudomonas (P. aeruginosa, P.otitidis), Staphylococcus (S. haemolyticus, S. sciuri, S. cohnii, S.xylosus, S. hominis, S. aureus), Rothia nasimurium, Streptococcus (S. pluranimalium, S. dysgalactiae) Achromobacter xylosoxidans, Comamonas kerstersii and Aerococcus viridians in nasal swabs of cattle with acute respiratory distress and Stapylococcus haemolyticus and Corynebacterium stationis in the blood samples of cattle. The results of the present study indicated that LSD could be one of the risk factors predisposing the cattle population to bacterial pathogens, which had led to respiratory diseases with high mortality among the cattle population of this region.