Polymorphism Analysis of spa Gene of Staphylococcus aureus isolated from Bovine Mastitis

Abidali Bhagat; H. N. Kher; A. I. Dadawala; H. C. Chauhan; M. D. Shrimali; B. S. Chandel

Authors

Abidali Bhagat Department of Microbiology, College of Basic Science and Humanities, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385 506, Gujarat, INDIA https://orcid.org/0000-0002-5847-6912
H. N. Kher Department of Veterinary Microbiology/Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385 506, Gujarat, INDIA
A. I. Dadawala Department of Microbiology, Kamdhenu University, Gujarat, INDIA
H. C. Chauhan Department of Veterinary Microbiology/Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385 506, Gujarat, INDIA
M. D. Shrimali Department of Veterinary Microbiology/Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385 506, Gujarat, INDIA
B. S. Chandel Department of Veterinary Microbiology/Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385 506, Gujarat, INDIA

Keywords:

Bovine Mastitis, PCR, Staphylococcus aureus, spa Gene

Abstract

Protein A is an important virulence factor of Staphylococcus aureus encoded by the spa gene. The presence of repeatable areas and the highly polymorphic nature of the spa gene, make it a good tool for epidemiologic investigation. The present study was undertaken to determine the spa gene variation in Staphylococcus aureus. A total of 55 S. aureus strains were isolated from cattle and buffaloes with clinical and subclinical mastitis. All the isolates were tested for the presence of spa gene of which 32 (58.18 %) isolates were found positive. Variable amplicon sizes of 160, 243, 270, 296, and 306 bp with calculated numbers of 5, 8, 10, 10 and 11 repeats were detected among 2 (6.25 %), 9 (28.13 %), 8 (25.00 %), 2 (6.25 %) and 11 (34.38 %) isolates, respectively. Furthermore, based on the calculated number, 52.63 percent of cattle Staphylococcus aureus and 58.82 percent of buffalo Staphylococcus aureus have more than 7 repeats. In conclusion, polymorphism of the spa gene was observed in S. aureus isolated from clinical and subclinical mastitis.