Cultural Characterization of Salmonella spp Isolated from Poultry Samples

Haritha  Routhu; Vijaya Kumar Anumolu; Sujatha Singh; Shashi M  Kumar; Krishnaiah N

doi:10.5455/ijlr.20191011100139

Authors

Haritha Routhu Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Rajendranagar, PV Narasimha Rao Telangana Veterinary University, Hyderabad-500030, Telangana, INDIA
Vijaya Kumar Anumolu Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Rajendranagar, PV Narasimha Rao Telangana Veterinary University, Hyderabad-500030, Telangana, INDIA
Sujatha Singh Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Rajendranagar, PV Narasimha Rao Telangana Veterinary University, Hyderabad-500030, Telangana, INDIA
Shashi Kumar M Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Rajendranagar, PV Narasimha Rao Telangana Veterinary University, Hyderabad-500030, Telangana, INDIA
Krishnaiah N Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Rajendranagar, PV Narasimha Rao Telangana Veterinary University, Hyderabad-500030, Telangana, INDIA

DOI:

https://doi.org/10.5455/ijlr.20191011100139

Keywords:

Egg Shells and Cloacal Swabs, Prevalence, Poultry Meat, Salmonella spp

Abstract

The prevalence of Salmonella spp in poultry (meat, egg and cloacal swabs) samples collected in and around
Hyderabad by cultural and the Polymerase Chain Reaction (PCR) methods. A total of 150 poultry samples
(50 each of meat, egg and cloacal swabs) were tested of which 45 and 50 samples were positive for
Salmonella spp by cultural method and PCR assay accounting for 30% and 33.3% respectively. Out of 50
poultry meat samples 17 samples (34%) were positive for Salmonella spp by cultural method, whereas
19(38%) were positive by PCR assay. Out of 50 each of egg shell and cloacal swab samples 15(30%) and
13(26.0%) respectively were positive by cultural method whereas 17(34%) and 14(28%) respectively were
positive by PCR assay.