Histomorphological Evaluation of Medial Patellar Ligament Affected with  Upward Luxation of Patella in Cattle

P. B. Chougale; R. V. Suryawanshi; A. H.  Ulemale; S. B. Lambate; C. S. Mote; A. B. Mali; Y. B.  Jadhav

doi:10.5455/ijlr.20191117100835

Authors

P. B. Chougale Department of Veterinary Surgery, Krantisinh Nana Patil College of Veterinary Science, Shirwal- 412801, Dist. Satara, Maharashtra Animal & Fishery Sciences University, Nagpur, Maharashtra, INDIA
R. V. Suryawanshi Department of Veterinary Surgery, Krantisinh Nana Patil College of Veterinary Science, Shirwal- 412801, Dist. Satara, Maharashtra Animal & Fishery Sciences University, Nagpur, Maharashtra, INDIA
A. H. Ulemale Department of Veterinary Surgery, Krantisinh Nana Patil College of Veterinary Science, Shirwal- 412801, Dist. Satara, Maharashtra Animal & Fishery Sciences University, Nagpur, Maharashtra, INDIA
S. B. Lambate Department of Veterinary Anatomy and Histology, Krantisinh Nana Patil College of Veterinary Science, Shirwal- 412801, Dist. Satara, Maharashtra Animal & Fishery Sciences University, Nagpur, Maharashtra, INDIA
C. S. Mote Department of Veterinary Pathology, Krantisinh Nana Patil College of Veterinary Science, Shirwal- 412801, Dist. Satara, Maharashtra Animal & Fishery Sciences University, Nagpur, Maharashtra, INDIA
A. B. Mali Department of Veterinary Gynecology and Obstetrics, Krantisinh Nana Patil College of Veterinary Science, Shirwal- 412801, Dist. Satara, Maharashtra Animal & Fishery Sciences University, Nagpur, Maharashtra, INDIA
Y. B. Jadhav Veterinary Officer

DOI:

https://doi.org/10.5455/ijlr.20191117100835

Keywords:

Collagen, Cattle, Electron microscopy, Heterogenecity, Luxation and Medial Patellar Ligament

Abstract

Clinical study was carried on twenty cattle aged between 6-13 years (6.25±0.59 years) presented with chief
complaint of upward luxation of patella to investigate this musculoskeletal malady in dairy animals.
Affected medial patellar ligament was harvested via open desmotomy whereas normal ligament was also
collected from animals that died due to automobile accident for comparative purpose and preserved in
2.5% glutaraldehyde solution. Histopathological examination of affected ligament showed degenerative
changes characterized by unevenly distributed and broken collagen fibers, atrophy and hypertrophied
collagen fiber with loss of nuclei indicative cellular alterations. Scanning electron microscopy of affected
ligament showed derangement in collagen fiber bundles arranged irregularly, wavy, loose and remarkable
gap between two collagen fiber bundles was noted. Transmission electron microscopy showed thin and
disorganized collagen fiber bundles with heterogenecity of size and diameter in collagen fiber bundles. To
concludes, affected medial patellar ligament undergoes several degenerative changes in collagen fibrils
that lead to changes in structure – function relationship causes luxation of patella in cattle.