Lymphocyte Proliferation Response of Blood and Milk Lymphocytes of Crossbred Cows During Peripartum Period

Krupa R.  Joshi; M. M. Pathan; S. P. Madhira; A. M. Pande; Dhara D. Dhusa

doi:10.5455/ijlr.20200731055404

Authors

Krupa R. Joshi P.G. Scholar, Department of Veterinary Physiology & Biochemistry, College of Veterinary Sci. & A. H., AAU, Anand, Gujarat, INDIA
M. M. Pathan Assistant Professor, Department of Veterinary Physiology & Biochemistry, College of Veterinary Sci. & A. H., AAU, Anand, Gujarat, INDIA https://orcid.org/0000-0002-8352-9975
S. P. Madhira Assistant Professor, Department of Veterinary Physiology & Biochemistry, College of Veterinary Sci. & A. H., AAU, Anand, Gujarat, INDIA
A. M. Pande Professor & Head, Department of Veterinary Physiology & Biochemistry, College of Veterinary Sci. & A. H., AAU, Anand, Gujarat, INDIA
Dhara D. Dhusa P.G. Scholar, Department of Veterinary Physiology & Biochemistry, College of Veterinary Sci. & A. H., AAU, Anand, Gujarat, INDIA

DOI:

https://doi.org/10.5455/ijlr.20200731055404

Keywords:

Cow, LPR, Lymphocyte, Peripartum Period

Abstract

Six crossbred cows (Holstein Fresian × Kankrej) were selected in their advanced stage of gestation. The study was conducted on the selected animals from 30 days before calving to 30 days after calving. From each experimental animal 9 ml Blood was drawn in sterile heparinized vacutainer tube from jugular vein, on days -30, -15, -7 and -3 before calving, on the day of calving and on days 3, 7, 15 and 30 after calving. From each experimental animal 25ml milk sample was also collected on the day of calving and 3, 7, 15, 30 days after calving. Highest lymphocyte count observed at 30 day before calving, whereas significant (p<0.05) reduction was observed on the day of calving. Lowest lymphocyte proliferation response (LPR) of blood was observed at 3rd day before calving while LPR of milk was observed highest on the day of calving. Highest blood plasma cortisol and prolactin was observed at calving.