Pathology and Molecular Diagnosis of Chicken Infectious Anemia in Commercial Layers of Chhattisgarh

Authors

  • Prashant M. Sonkusale Assistant professor, Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
  • Ratan Chnadra Ghosh Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
  • Devesh Kumar Giri Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
  • Vivek Kumar Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
  • Poornima Gumasta Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
  • Neha Shukla Department of Pathology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA
  • Savita Bisen Department of Parasitology, College of Veterinary Science & A. H., Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh, INDIA

Keywords:

CIA, Commercial Layers, Molecular Diagnosis, Histopathology

Abstract

Poultry is constantly exposed to various immunosuppressive agents such as viruses, mycotoxins, and environmental stress. Chicken Infectious Anemia (CIA) caused by a Circovirus is one of the very important viral diseases. During the present study, mortality of 15 to 25% due to Chicken infectious anemia in eleven, 6-11weeks old commercial layer flocks located at Durg, Rajnandgaon, and Raipur districts of Chhattisgarh was noticed which were not vaccinated against CIA. Characteristic clinical signs of anemia indicated by pale comb and wattle, yellowish changes in the bone marrow, and thymic atrophy were suggestive of CIA. Generalized lymphoid atrophy was the most constant and characteristic lesion found in CIA-affected birds. Clinical samples of thymus collected from eleven commercial layer flocks were confirmed as CIA by PCR amplification of 419bp of the VP2 gene of the virus. The clinical signs, gross and histopathological findings along with PCR amplification of the VP2 gene confirmed the outbreak of CIA in the commercial layer.

References

Baksi, S., Savaliya, B.F., Rao, N. and Khan, M. (2016) Sero-Prevalence of Chicken anemia virus (CAV) in layer poultry flocks in different parts of India. Journal of Poultry Science and Technology, 4(3):39-42.

Bhatt, P., Shukla, S.K., Mahendran, M., Dhama, K., Chawak, M.M. and Kataria, J.M. (2011) Prevalence of chicken infectious anaemia virus (CIAV) in commercial poultry flocks of northern India: A serological survey. Trans boundary Emerging Disease. 58: 458 – 460.

Chandrashekaraiah, B., Karamala, S., Narayanaswamy, H. D., Karumuri, N.K. and Nagaram, V.K. (2020) Pathology of chicken infectious anemia (CIA) with concurrent infections. Journal of Entomology and Zoology Studies, 8(2): 519-524

Davidson, M., Kedem, H., Borochovitz, N., Kass, G., Ayali, E. and Hamzani, B. (2004) Chicken infectious anemia virus infection in Israeli commercial flocks: virus amplification, clinical signs, performance and antibody Status. Avian Disease, 48(1):108-118

Dhama, K., Mahendran, M., Somvanshi, R. and Chawak, M.M. (2008) Chicken infectious anemia virus: an immunosuppressive pathogen of poultry – A review. Indian Journal of Veterinary Pathology, 32: 158 – 167

Gowthaman, V., Singh, S.D., Dhama, K., Barathidasan, R., Srinivasan, P. and Mahajan, N.K. (2014) Molecular characterization of chicken infectious anemia virus isolated from commercial poultry with respiratory disease complex in India. Advances in Animal and Veterinary Science, 2(3):171-176

Hoop, R.K., Guscetti, F. and Keller, B. (1992) An outbreak of infectious chicken anemia in fattening chickens in Switzerland. Schweizer Archiv fur Tierheilkunde 134(10):485- 489.

Khanna, S. K. (2010) Emerging diseases of poultry: newer and cheaper methods of diagnosis and treatment. Poultry Technology, 5: 68 – 71.

Ledesma, N., Fehervari, T., Casaubon, M., Eduardo, L. and Ratz, F. (2001) Chicken infectious anemia in Mexico: Virus identification and serology survey. Avian Disease, 45(4):788-796.

Luna, A. G. (1968) Manual of histology and special staining technique. 3rd Ed, 1, Armed Force Institute of Pathology-Magraw Hill Book Co, London.

Narayani, A. and Ghosh, R. C. (2018) Studies on pathogenicity of Chicken infectious anaemia virus isolated from poultry flock of Chhattisgarh. Indian Journal of Animal Research, 8(3):361-366.

Natesan, S., Kataria, J.M., Dhama, K., Rahul, S. and Baradhwaj, N. (2006) Biological and molecular characterization of Chicken anemia virus isolates of Indian origin. Virus Research, 118: 78 – 86.

Ottiger, H.P. (2010) Development, standardization and assessment of PCR system for purity testing of avian viral vaccine. Biologicals, 38: 381 –388

Praveen, B.N., Dhama, K., Kataria, J.M., Dash, B.B. and Singh, S.D. (2008) Detection and isolation of Chicken anemia virus from field sample of poultry flocks from Gujarat and Andhra Pradesh, India. Indian Journal of Comparative Microbiology Immunology and Infectious Diseases. 29: 23 – 26

Rimondi, A., Pinto, S., Olivera, V., Diborbora, M., Perez-Filgueira, M. and Craig, M.I. (2014) Comparative, histopathological and immunological study of two field strains of chicken anemia virus. Veterinary Research, 45:1-12

Todd, D. (2004) Avian circovirus diseases: lessons for the study of PMWS. Veterinary Microbiology, 98: 169 –174

Toro, H.S., Ewald, A. and Hoerr, F.J. (2006) Serological evidence of chicken infectious anemia virus in the United States. Avian Disease, 50: 124 – 126.

Wani, M.Y., Dhama, K., Barathidasan, R., Gowthaman, V., Tiwari, R., Bhatt, P., Mahajan N.K., Chawak, M.M., Singh, S.D. and Kataria, J.M. (2013) Molecular detection and epidemiology of chicken infectious anaemia virus in India. South Asian Journal of Experimental Biology. 3(4): 145 ‐ 151.

Yao, S., Tuo, T., Gao, X., Han, C., Yan, N. and Liu, A. (2019) Molecular epidemiology of chicken anaemia virus in sick chickens in China from 2014 to 2015. PLoS One.14(1): e0210696. doi: 10.1371/journal.pone.0210696.

Downloads

Published

31-12-2022

How to Cite

Sonkusale, P. M., Ghosh, R. C., Giri, D. K., Kumar, V., Gumasta, P., Shukla, N., & Bisen, S. (2022). Pathology and Molecular Diagnosis of Chicken Infectious Anemia in Commercial Layers of Chhattisgarh. International Journal of Livestock Research, 12(12), 13–17. Retrieved from https://ijlr.org/ojs_journal/index.php/ijlr/article/view/159

Most read articles by the same author(s)