Molecular Diagnosis of Bovine Dermatophilosis Using Species Specific Polymerase Chain Reaction

Abstract

Present study involves use of a species specific PCR for molecular confirmation of D. congolensis isolates from cattle in Kerala. Thirty isolates of Dermatophilus congolensis isolated from dermatitis cases of cattle from Kerala were used in the study. Genomic DNA was extracted using the High Pure PCR Template preparation Kit. A species specific PCR to detect D. congolensis based on a 1029 bp internal, conserved region of the alkaline ceramidase gene was carried out using specific primers,  5’- GCG TAA CCG CTC CCT TCC TGC TCT TCC GCT TCC TC-3’ (DCACIF) and  5’-GCC AAA CCA GCA GCC TCT TGC CGC ATA CAC TAT TCT C-3’   (DCACIR). The use of this species specific PCR for molecular confirmation of D. congolensis isolates was found to be highly specific in detecting D. congolensis by discriminating from other bacteria.