Ovarian and Fertility Responses Following Administration of GnRH During Estrus and PGF2α during Diestrus in Repeat Breeder Cattle
Keywords:
Cattle, GnRH, PGF2α, Repeat BreedingAbstract
The study was conducted on crossbred dairy cows (n=30) presented at Veterinary Clinical Complex, with a history of repeat breeding. The study compared ovarian and fertility responses in such animals while using exogenous Gonadotropin Releasing Hormone GnRH, Prostaglandin F (PGF2α), and double Artificial Insemination (AI) in three groups viz; Group I- (n=10), administered GnRH (Buserelin Acetate @ 20µg total dose) at spontaneous estrus followed by double AI, Group II (n=10)- administered PGF2α (Cloprostenol Sodium@ 500µg total dose) on day 12 of the cycle, followed by double AI at induced estrus and Group III (n=10) subjected to double AI without any hormonal drug, at spontaneous estrus. The second AI performed 18 hrs of the first insemination in all three groups. Ovulation and conception rates were significantly higher and the duration between mid-estrus (mounting and copious mucus discharge) to ovulation was shorter in Group I (p<0.05), compared to Groups II and III. In conclusion, the use of GnRH at the time of estrus in repeat breeder cows is more efficient in terms of inducing faster ovulation with higher ovulation and conception rates. Also breaking the estrus cycle during the luteal phase by PGF2α, followed by double insemination using frozen semen has an advantage over double insemination in repeat breeders.
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