The aim of the present study was to evaluate the efficacy of supplementation of herbal choline as a replacement of synthetic choline chloride in diets on performance of broiler chickens. Three hundred straight-run Cobb-400 day-old broiler chicks were randomly distributed into five treatment groups having three replicates having 20 chicks in each and reared up to 6 weeks of age. The treatment group T1 (control) offered basal diet (BIS, 2007) without synthetic choline chloride-60% (CC) or herbal choline (HC), group T2 basal diet with choline chloride-60% @ 1 kg/ton of feed, groups T3, T4 and T5 offered basal diets with herbal choline @ 0.250, 0.350 and 0.500 kg/ton of feed, respectively. The weekly growth performance parameters were studied up to 6th week of age in broiler chickens. The mean weekly body weight and weight gain of birds in group T5 group (0.5 kg/ton herbal choline) was significantly (p<0.05) higher than all treatment groups. Whereas, the statistically significant (p<0.05) higher mean weekly body weight and weight gain was observed in treatment groups T2 and T4 as compared to control group T1 fed diet without supplementation of any choline chloride. The birds from treatment group T5 fed diet containing herbal choline at 0.500 kg/ton of feed showed significantly higher (p<0.05) cumulative feed consumption than treatment group T2 fed diet at 1 kg/ton of synthetic choline. The treatment groups T2, T4 and T5 showed significantly better (p<0.05) cumulative feed conversion ratio than groups T1 and T3 (herbal choline at 0.250 kg/ton of feed). The higher survivability was observed in groups T2 and T5 compared to all the treatment groups. The highest mortality was observed in control group T1 fed diets without supplementation of synthetic choline chloride or herbal choline in feed. The post mortem examination from group T1 revealed specific lesions which indicated that the birds were died due to fatty liver syndrome. The highest EPEF was observed in treatment group T5 receiving diet at 0.500 kg/ton of herbal choline followed by treatment groups T2, T4, T3 and T1. The overall performance of the birds under treatment group T5 was found to be better. It may be concluded that supplementation of herbal choline at 0.350 and 0.500 kg/ton of feed replaces synthetic choline chloride-60% routinely added at 1 kg/ton of broiler feed. Moreover, the supplementation of herbal choline at 0.500 kg/ton of feed was more beneficial in terms of improving the bird’s performance as revealed by European performance efficiency factor.