The present study was carried out to evaluate efficacy of Diminazene aceturate along with erythropoietin to ameliorate trypanosome induced anaemia and osmotic fragility of erythrocytes (EOF). Twenty four adult mice were randomly divided into four groups of six animals each. All animals except those in uninfected control group were infected with Trypanosoma evansi and treated with Diminazene aceturate and/or human recombinant erythropoietin. At the end of three weeks whole blood was collected in EDTA to evaluate EOF and level of anaemia. Result of this study established a significantly higher EOF in infected controls compared to treatment group and development normocytic normochromic anaemia progressing to microcytic hypochromic anaemia.