The Effect of Supplementation of Oregano Oil and Probiotic on Intestinal Microbes (Namely E. coli spp., Salmonella spp., Clostridia spp.) of the Broiler Chicken

Harshal N. Rewatkar; S. M. Wankhede; J. L. Agashe; R. M. Padole; A. D. Jadhao; G. M. Jadhao

Authors

Harshal N. Rewatkar Department of Animal Nutrition, Post Graduate Institute of Veterinary and Animal Sciences, Akola, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra, INDIA
S. M. Wankhede Department of Animal Nutrition, Post Graduate Institute of Veterinary and Animal Sciences, Akola, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra, INDIA
J. L. Agashe Department of Poultry Science, Post Graduate Institute of Veterinary and Animal Sciences, Akola, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra, INDIA
R. M. Padole Department of Pathology, Post Graduate Institute of Veterinary and Animal Sciences, Akola, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra, INDIA
A. D. Jadhao Department of Animal Nutrition, Post Graduate Institute of Veterinary and Animal Sciences, Akola, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra, INDIA
G. M. Jadhao Department of Animal Nutrition, Post Graduate Institute of Veterinary and Animal Sciences, Akola, Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra, INDIA

Keywords:

Broiler, Intestinal Microbes, Oregano Essential Oil, Probiotic

Abstract

To evaluate the effect of oregano oil and probiotic on total microbial count of intestine namely E. coli, Salmonella and Clostridia. On 42^nd day, 8 birds from each treatment were slaughtered by decapitation. The digestive tracts were dissected and described Caecal content samples were taken aseptically and were transferred into sterile plastic bags and immediately transported in cold chain to the laboratory, samples diluted, cultured and checked for the presence of organisms in samples (E. coli, Salmonella, Clostridia) and compared with the control after incubation. Results were expressed as log10 colony-forming units per gram of ileal digesta (log10 CFU/gram). The intestinal microbial count namely E. coli, Salmonella, Clostridia were significantly decreased in T₃than in groups T₂, T₁ and control. It was concluded that feeding of Oregano essential oil and probiotic- Saccharomyces cerevisiae exhibited beneficial effect on GIT and decreased the harmful microbial count of the intestine in the broiler chicken.