This two year prospective study was performed to determine the clinical signs and haemato-biochemical alterations in eleven buffaloes suffering from caecal dilatation, in hospital admitted population of 357 buffaloes which were confirmed to have gastrointestinal dysfunction. Ten clinically healthy buffaloes were considered as control. The diagnosis of caecal dilatation was made on the basis of physical examination, after ruling out other gastrointestinal disorders. Complete anorexia, colic, constipated faeces, reduced to nil rumen motility, mild to moderate dehydration, distension of right flank and ping sounds on simultaneous auscultation and percussion of right paralumbar fossa were the most pronounced clinical symptoms. Affected buffaloes had significantly higher neutrophil count and lower lymphocytic count than control group. Biochemical analysis showed significant increase of total bilirubin, AST, globulin, lactate and blood urea nitrogen levels and significant reduction in calcium, potassium and chloride. In conclusion, caecal dilatation produces alterations in the clinical, haematological and biochemical picture compared to control healthy buffaloes.