Brucellosis is the widely prevalent disease throughout India causing economic losses to the tune of Rs. 350 million. The current status of bovine brucellosis in India is not clear. However, the incidence of the disease is increasing in all the states of the country. Scanty literature is available on bubaline brucellosis from Malwa region of Madhya Pradesh, therefore study was conducted to analyse the haematological changes in infected animals. Samples were collected from 150 buffaloes (both lactating and non-lactating) from slaughter house and nearby areas of Mhow and Indore. Out of 150 serum samples, 20 serum samples were found positive for Rose Bengal Plate Test (RBPT). Out of 18 milk samples, 7 milk samples were found positive for Milk Ring Test (MRT) and 10 samples found positive for California mastitis test (CMT). Haematological studies revealed decrease in neutrophil, monocyte, eosinophil, lymphocyte and total erythrocyte counts in RBPT positive while increase in haemoglobin, packed cell volume and total ertythrocyte count in MRT and CMT positive animals.