This study was undertaken in field conditions on 50 cyclic repeat breeder and 50 acyclic buffaloes to evaluate the efficacy of four standard estrus induction/ synchronization protocols, viz., Doublesynch (PG-GPG), Estradoublesynch (PG-GPE), Ovsynch (GPG), and Ovsynch Plus (eCG-GPG) (10 buffaloes in each protocol, and in controls) in terms of estrus induction response and conception rates at induced estrus with FTAI and overall of 3 cycles post-treatment. All repeat breeder and 90-100 % acyclic buffaloes under four protocols exhibited induced estrus within mean intervals of 69.80±3.44, 62.10±2.84, 61.45±1.34 and 64.00±1.25 h, and within 64.77±2.19, 62.50±1.39, 69.30±1.80 and 70.60±1.30 h, respectively, from PGF2α injection. The conception rates obtained in cyclic/repeat breeder buffaloes at induced estrus (FTAI) and overall of three cycles post-treatment were 40 and 50 % with Doublesynch; 50 and 70 % with Estradoublesynch; 60 and 70 % with Ovsynch, and 60 and 60 % with Ovsynch Plus protocol, respectively. The corresponding conception rates in acyclic buffaloes were 50 and 60 %; 40 and 60 %; 30 and 80 %, and 50 and 70 %. Among 10 repeat breeder buffaloes each, 4, 3, 2 and 3 non-conceived buffaloes remained cyclic, while 1, 0, 1 and 1 became anestrus by 60 days post-induction/FTAI following use of Doublesynch, Estradoublesynch, Ovsynch and Ovsynch Plus protocols, respectively, whereas among 10 acyclic buffaloes each, 2, 2, 2 and 1 non-conceived buffaloes remained cyclic, while 2, 2, 0 and 2 again turned out to be anestrus by 60 days follow up. Among the untreated control cyclic and acyclic buffaloes (10 each), 3 and 2 buffaloes conceived over a period of 90 days follow up giving overall conception rates of only 30 and 20 %, respectively, as against 62.5 and 67.5 % in treated cyclic and acyclic buffaloes. It was thus concluded that all four protocols improved conception rates in repeat breeding and acyclic buffaloes by establishing regular cyclicity post-treatment. The maximal benefit was with Ovsynch and/or Estradoublesynch application in repeat breeders and Ovsynch and/or Ovsynch Plus in anestrus buffaloes.