Anti-Oxidant Effect of Quercetin in Tris Egg Yolk Citrate Extender on Surti Buck Semen Preserved at Refrigerated Temperature

W. V. Diniz; L. C. Modi; N. F. Chaudhari; Sandhya Chaudhary; M. A. Pandor

doi:10.5455/ijlr.20201016043822

Authors

W. V. Diniz M. V. Sc. Scholar, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, INDIA
L. C. Modi Assistant Professor, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, INDIA
N. F. Chaudhari Assistant Professor, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, INDIA
Sandhya Chaudhary Professor & Head, Department of Veterinary Physiology & Biochemistry, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, INDIA
M. A. Pandor M. V. Sc. Scholar, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, INDIA

DOI:

https://doi.org/10.5455/ijlr.20201016043822

Keywords:

Antioxidant, Buck Semen, MDA, Quercetin, Refrigerated Temperature, SOD

Abstract

A study was conducted to examine antioxidant effect of quercetin in tris egg yolk citrate extender on chilling quality of Surti buck semen. Total 72 semen ejaculates were collected from four Surti buck (18 ejaculate/buck) twice in a week by artificial vagina method. Semen samples were diluted with Tris egg yolk citrate extender and quercetin at different concentration viz. 0 µM/ml, 15 µM/ml, 25 µM/ml, 50 µM/ml, 75 µM/ml, 100 µM/ml and stored at refrigerated temperature (4-5 ºC). Evaluation of semen parameters was done at 0 and 48 hours. The result exhibited lower MDA level in 15 µM/ml quercetin added in tris egg yolk citrate extender group compared to control group in buck semen preserved at refrigerated temperature up to 48 hours. Marginally higher SOD activity was observed in 15 µM/ml quercetin group as compared to other treatment and control groups. Moreover, SOD values were observed to be decreasing with increase in quercetin concentration at both the time intervals.

References

Aherne, S. A. and O Brien, N. M. (2000). Mechanism of protection by the flavonoids, quercetin and rutin, against tert-butyl hydroperoxide‐ and menadione induced DNA single strand breaksin Caco-2 cells. Free Radical Biology and Medicine, 29: 507–514.

Aurich, J. E., Schonherr, U., Hoppe, H. and Aurich, C. (1997). Effects of antioxidants on motility and membrane integrity of chilled-stored stallion semen. Theriogenology, 48: 185–192.

Avdatek, F., Yeni, D., Inanc, M. E., Cil, B., Tuncer, B. P., Turkmen, R. and Tasdemir, U. (2018). Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation. Andrologia, 50(4): 129-75.

Beltran, M. A. G., Atabay, E. P., Atabay, E. C., Cruz, E. M., Aquino, F. P. and Cruz, L. C. (2013). Optimized extenders for cryopreservation of buck semen for artificial insemination. Philippine Journal of Veterinary and Animal Sciences, 39(1): 1-10.

Ben Abdallah, F., Zribi, N. and Ammar-Keskes, L. (2011). Antioxidative potential of Quercetin against hydrogen peroxide induced oxidative stress in spermatozoa in vitro, Andrologia, 43: 261-265.

Boots, A. V., Haenen, G. R. and Bast, A. (2008). Health effects of quercetin: From antioxidant to nutraceutical. European Journal of Pharmacology, 585: 325–337.

Ferdinand, N., Thomas, T. T., Augustave, K., Henry, D. F., Fernand, T. and Etienne, P. T. (2012). Effects of Buck Age, Storage Duration, Storage Temperature and Diluent on Fresh West African Dwarf Buck Semen. Journal of Reproduction and Infertility, 3(3): 58-66.

Forman, H. J. and Torres, M. (2002). Reactive oxygen species and cell signaling: respiratory burst in macrophage signaling. American Journal of Respiratory and Critical Care Medicine, 166: 4–8.

Ghaniei, A., Eslami, M., ZadehHashem, E., Rezapour, R. and Talebi, A. (2019). Quercetin attenuates H2O2‐induced toxicity of rooster semen during liquid storage at 4° C. Journal of Animal Physiology and Animal Nutrition, 103(3): 713-722.

Johinke, D., De Graaf, S. P. and Bathgate, R. (2014). Quercetin reduces the in vitro production of H2O2 during chilled storage of rabbit spermatozoa. Animal Reproduction Science, 151(3-4): 208-219.

Jones, R. and Mann, T. (1977). Toxicity of exogenous fatty acid peroxide towards spermatozoa. Journal of Reproduction and Fertility, 50: 225-260.

Kelly, G. S. (2011). Quercetin. Alternative Medicine Review, 16: 172-194.

Khaki, A., Fathiazad, F., Nouri, M., Khaki, A., Maleki, N. A., Khamnei, H. J. and Ahmadi, P. (2010). Beneficial effects of quercetin on sperm parameters in streptozotocin‐induced diabetic male rats. Phytotherapy Research, 24(9): 1285-1291.

Khanduja, K. L., Verma, A. and Bhardwaj, A. (2001). Impairment of human sperm motility and viability by quercetin is independent of lipid peroxidation. Andrologia, 33: 277–281.

Kumaran, A. and Karunakaran, R. J., (2006). Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chemistry, 97: 109–114.

Madesh, M. and Balasubramanian, K. A. (1998). Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian Journal of Biochemistry & Biophysics, 35(3): 184-188.

Mann, T., Jones, R. and Sherins, R. (1980). Oxygen damage, lipid peroxidation and motility of spermatozoa. In: Steinberg, A., Steinberg E., editors. Testicular Development, Structure and Function. Raven Press: New York, USA pp. 497-501

Mazzilli, F., Rossi, T., Sabatini, L., Pulcinelli, F. M., Rapone, S., Dondero, F. and Gazzaniga, P. P. (1995). Human sperm cryopreservation and reactive oxygen species (ROS) production. Acta Europaea Fertilitatis, 26: 145-148.

Nogueira, B. G., Bitencourt, J. L., Sampaio, B. F. B., Bender, E. S. C., Costa e Silva, E. V. and Zuccari, C. E. S. N. (2013). Rev 48 Electron Vet. 15: 1-15.

Rehman, S. U. (1984). Lead induced regional lipid peroxidation in brain. Toxicology Letters, 21: 333-337.

Seifi-Jamadi, A., Ahmad, E., Ansari, M. and Kohram, H. (2017). Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen. Cryobiology, 75: 15-20.

Seifi-Jamadi, A., Kohram, H., Shahneh, A. Z., Ansari, M. and Macías-García, B. (2016). Quercetin ameliorate motility in frozen-thawed turkmen stallions sperm. Journal of Equine Veterinary Science, 45: 73-77.

Tremellen, K. (2008). Oxidative stress and male infertility a clinical perspective. Human Reproduction Update, 14: 243–258.

Yelumalai, S., Giribabu, N., Kamarulzaman Karim, S. Z. O. and Salleh, N. B. (2019). In vivo administration of quercetin ameliorates sperm oxidative stress, inflammation, preserves sperm morphology and functions in streptozotocin-nicotinamide induced adult male diabetic rats. Archives of Medical Science, 15(1): 240.