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Effect of Butylated Hydroxytoluene on Acrosome Integrity and Viability of Crossbred Bull Spermatozoa

Anoop Singh Mridula Sharma Shiv Prasad Yaqoob Bhat Anil Kumar Dinesh Pandey Santosh Shukla
Vol 7(7), 82-91

The present study was conducted to observe the effect of Butylated hydroxytoluene (BHT) on acrosomal integrity of crossbred bovine bull spermatozoa. Total eighteen ejaculates from two crossbred bulls were collected and extended with egg yolk tris dilutor along with addition of different concentrations of BHT i.e. 0.0mM (Control), 0.5mM (T1) and 1.0mM (T2), respectively. Acrosome integrity was assessed by Trypan blue giemsa staining during post dilution (Stage I), post equilibration (Stage II) and post thaw (Stage III). Mean percent of AIL spermatozoa were significantly (p<0.05) higher in group T2 compared to T1 and control at stage I and II. At stage III, T1 and T2 showed significantly (p<0.05) higher AIL sperms compared to control. Mean percent of AID spermatozoa were significantly (p<0.05) higher in control compared to T1 and T2 at Stage I, II and III. T2 showed significantly lower ALL and ALD spermatozoa compared to control at stage III. The study indicates that 1mM BHT increased the cryosurvival i.e., (higher acrosomal integrity and viability) i.e. higher AIL and lower AID, ALL and ALD spermatozoa.

Keywords : Semen Crossbred Bull BHT Trypan Blue/Giemsa Acrosomal Integrity and Viability

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