The study was conducted to assess the combined effects of herbs on semen quality and oxidative stress in subfertile buffalo bulls (Bubalus bubalis). A total of 144 semen ejaculates (16 ejaculates/bull) were collected from 3 subfertile buffalo bulls following oral supplementation of Panax ginseng roots, Shilajit, Withania somnifera roots, Tribulus terrestris fruits, Turnera diffusa leaves, Ptychopetalum olacoides bark each @ 400 mg/100 kg body weight and Pausinystalia yohimbe bark @ 300 mg/100 kg body weight of bulls for 60 days. Semen was evaluated for quality and oxidative stress (lipid peroxidation, superoxide dismutase and glutathione peroxidase) during pre-supplementation, supplementation and post-supplementation phases (each phase of 60 days). Herbal supplementation significantly (P<0.05) improved mass motility, individual motility, rapid progressive motility, curvilinear velocity, sperm concentration, viability and active mitochondria. Total sperm abnormalities and amplitude of lateral head displacement were significantly (P<0.05) reduced during supplementation and post-supplementation phases. The level of malondialdehyde was significantly (P<0.05) declined during supplementation and post-supplementation phases. The activity of superoxide dismutase significantly (P<0.05) increased during supplementation and post-supplementation phases, whereas the activity of glutathione peroxidase was significantly (P<0.05) increased only during the supplementation phase. It could be concluded that feeding of herbs in combination improved the semen quality and antioxidants activity in subfertile buffalo bulls.